PIPES Na / Sodium Hydrogen Piperazine-1 4-diethanesulphonate CAS 10010-67-0

1. Acts Sodium hydrogen piperazine-1 4-diethanesulphonate CAS 10010-67-0 as a zwitterionic Good's buffer in cell culture media to stabilize pH for mammalian cell proliferation and microbial fermentation.
2. Serves PIPES Na as a pH stabilizer in electron microscopy tissue fixation and enzyme activity assays to preserve biomolecular structure.
3. Functions as a buffer in protein electrophoresis and immunoprecipitation protocols for separating acidic and neutral proteins.

1. Store Sodium hydrogen piperazine-1 4-diethanesulphonate in sealed moisture-proof containers at 15-25℃ in a cool, dry, dark area away from strong acids and oxidizing agents.
2. Avoid PIPES Na prolonged exposure to heat and light to prevent decomposition and maintain consistent buffering performance.
3. Wear nitrile gloves, safety goggles, and a lab coat during handling to avoid skin contact and dust inhalation.

1. High Sodium hydrogen piperazine-1 4-diethanesulphonate water solubility enables easy preparation of concentrated buffer solutions for biological and biochemical experiments.
2. Minimal metal ion chelation makes it suitable for experiments involving metal-dependent enzymes and cell signaling pathways.
3. Low PIPES Na UV absorbance and chemical stability ensure compatibility with optical detection and long shelf life of prepared buffers.

- GHS Sodium hydrogen piperazine-1 4-diethanesulphonate Classification: Irritant (GHS07, Xi), causes skin irritation (H315), causes serious eye irritation (H319), may cause respiratory irritation (H335).
- Prolonged skin contact can lead to redness and itching; eye contact may result in tearing and temporary discomfort.
- Non-flammable PIPES Na and chemically stable under recommended storage conditions; avoid temperatures above 310℃ to prevent thermal decomposition.
- UN Number: Not listed as a hazardous substance for general transport; classified as a non-dangerous good under normal conditions.