Bis-Tris Propane CAS 64431-96-5

1. Bis-Tris propane is a zwitterionic biological buffer widely used in biochemistry and molecular biology to maintain stable pH across a broad range (pH 6.0-9.5), with pKa values of 6.8 and 9.0 at 25°C .
2. Bis-Tris propane CAS 64431-96-5 serves as a buffering agent in applications such as protein purification, enzymatic assays, and capillary electrophoresis, where its minimal metal ion binding and low toxicity are advantageous .
3. Bis-Tris propane is employed in studies of photosynthetic electron transport, in cell culture media formulations, and as a component in buffer systems for isoelectric focusing and chromatographic separations .
4. Researchers utilize Bis-Tris propane CAS 64431-96-5 as a reference standard in analytical chemistry and for maintaining consistent pH conditions in biological and biochemical experiments .

1. Bis-Tris propane must be stored in a tightly sealed container in a cool, dry, and well-ventilated environment, protected from light and moisture; recommended storage at room temperature in a cool, dark place below 15°C .
2. Bis-Tris propane CAS 64431-96-5 is classified with GHS hazard statements H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation), with signal word "Warning" .
3. Personnel must wear chemical-resistant gloves, safety goggles with side-shields, and protective clothing when handling this substance, ensuring adequate ventilation in work areas .
4. Keep away from incompatible materials such as strong oxidizing agents to prevent decomposition; wash thoroughly after handling .

1. Isolate the spill area, ensure adequate ventilation, and wear full personal protective equipment including chemical-resistant gloves, safety goggles, and suitable dust respirator when Bis-Tris propane is released .
2. Collect Bis-Tris propane CAS 64431-96-5 by sweeping or vacuuming without creating dust using non-sparking tools, and place into suitable closed containers for disposal .
3. Avoid creating dust clouds during cleanup to prevent respiratory exposure; prevent the product from entering drains or water courses .
4. Dispose of collected material according to local, state, and federal environmental regulations through licensed chemical destruction or controlled incineration .

1. Bis-Tris propane is not classified as highly flammable but may decompose under fire conditions, emitting toxic fumes including carbon monoxide, carbon dioxide, and nitrogen oxides (NOx) .
2. Use water spray, dry chemical powder, carbon dioxide, or alcohol-resistant foam to extinguish fires involving the product; do NOT use a direct water jet as Bis-Tris propane CAS 64431-96-5 may scatter burning material .
3. Firefighters should wear self-contained breathing apparatus in pressure-demand, MSHA/NIOSH approved, and full protective gear when handling burning Bis-Tris propane due to hazardous decomposition gases generated by thermal decomposition .
4. During combustion, may emit irritant and toxic fumes; contain fire runoff to prevent environmental contamination .