Tricine CAS 5704-04-1

1. Tricine CAS 5704-04-1 is a zwitterionic buffering agent widely used in biochemistry and molecular biology for protein separation and purification, particularly in low-voltage electrophoresis systems for resolving small proteins and peptides .
2. Tricine serves as an effective buffer in the pH range of 7.4 to 8.8, with a pKa of 8.05 at 25°C, and is commonly employed in SDS-PAGE for the analysis of proteins smaller than 30 kDa .
3. Tricine is utilized in studies of photosynthetic electron transport and as a buffer in enzymatic assays, where its low ionic strength and minimal metal ion binding properties are advantageous .
4. In cell culture and cryopreservation, tricine is used as a component in stabilization buffers, and researchers employ it in analytical chemistry as a reference standard for capillary electrophoresis and other separation techniques .

1. Tricine CAS 5704-04-1 must be stored in a tightly sealed container in a cool, dry, and well-ventilated environment, protected from light and moisture; recommended storage at room temperature in a cool, dark place below 15°C .
2. Tricine is classified with GHS hazard statements H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation), with signal word "Warning" .
3. Personnel must wear chemical-resistant gloves, safety goggles with side-shields, and protective clothing when handling this substance, ensuring adequate ventilation in work areas .
4. Keep away from incompatible materials such as strong oxidizing agents to prevent decomposition; wash thoroughly after handling .

1. Isolate the spill area, ensure adequate ventilation, and wear full personal protective equipment including chemical-resistant gloves, safety goggles, and suitable dust respirator when Tricine is released .
2. Collect Tricine by sweeping or vacuuming without creating dust using non-sparking tools, and place into suitable closed containers for disposal .
3. Avoid creating dust clouds during cleanup to prevent respiratory exposure; prevent the product from entering drains or water courses .
4. Dispose of collected material according to local, state, and federal environmental regulations through licensed chemical destruction or controlled incineration .

1. Tricine is not classified as highly flammable but may decompose under fire conditions, emitting toxic fumes including carbon monoxide, carbon dioxide, and nitrogen oxides (NOx) .
2. Use water spray, dry chemical powder, carbon dioxide, or alcohol-resistant foam to extinguish fires involving the product; do NOT use a direct water jet as Tricine may scatter burning material .
3. Firefighters should wear self-contained breathing apparatus in pressure-demand, MSHA/NIOSH approved, and full protective gear when handling burning Tricine due to hazardous decomposition gases generated by thermal decomposition .
4. During combustion, may emit irritant and toxic fumes; contain fire runoff to prevent environmental contamination .