Proteinase K CAS 39450-01-6

1. Proteinase K is a broad-spectrum serine protease used in molecular biology for the digestion of proteins during nucleic acid purification, effectively removing contaminating enzymes like DNases and RNases .
2. Proteinase K CAS 39450-01-6 is employed in tissue lysis protocols for DNA and RNA extraction from biological samples, including mammalian tissues, cells, and microorganisms, due to its high activity even in the presence of denaturing agents such as SDS and urea .
3. Proteinase K is utilized in the inactivation of enzymes during the preparation of chromosomal DNA and in the degradation of unwanted proteins in assays, enabling downstream applications like PCR, restriction digestion, and sequencing .
4. Researchers use Proteinase K CAS 39450-01-6 in the detection of bacterial endotoxins and as a critical reagent in prion research for its ability to digest protein aggregates and differentiate between normal and pathological forms .

1. Proteinase K must be stored in a tightly sealed container at -20°C for long-term stability; it can be stored at 4°C for short-term use, but repeated freeze-thaw cycles should be avoided .
2. Proteinase K CAS 39450-01-6 is shipped and stored in its lyophilized or solution form; once reconstituted (e.g., in Tris-HCl buffer containing calcium chloride), it is stable for several months at 4°C .
3. Proteinase K is classified as an irritant with hazard statements H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation); Proteinase K CAS 39450-01-6 may cause allergic respiratory reactions in sensitive individuals .
4. Personnel must wear chemical-resistant gloves, safety goggles with side-shields, and protective clothing when handling this substance, working in a well-ventilated area (preferably a chemical fume hood) to avoid inhalation of aerosols or dust .

1. Isolate the spill area, ensure adequate ventilation, and wear full personal protective equipment including chemical-resistant gloves, safety goggles, and a suitable respirator when Proteinase K is released .
2. For small spills, carefully wipe up using absorbent paper and place into a suitable container for disposal; for larger spills, cover with inert absorbent material (diatomite, sand) and sweep into a closed container .
3. Avoid Proteinase K CAS 39450-01-6 creating aerosols during cleanup; decontaminate surfaces and equipment by wiping with a disinfectant or 70% ethanol .
4. Dispose of collected material according to local, state, and federal environmental regulations; the substance is typically inactivated by autoclaving before disposal as non-hazardous waste .

1. Proteinase K is not classified as flammable; however, it may decompose under fire conditions, emitting toxic fumes including carbon monoxide and carbon dioxide .
2. Use Proteinase K CAS 39450-01-6 water spray, dry chemical powder, carbon dioxide, or alcohol-resistant foam to extinguish fires involving the product .
3. Firefighters should wear self-contained breathing apparatus in pressure-demand, MSHA/NIOSH approved, and full protective gear when handling burning Proteinase K .
4. During combustion, may emit irritant and toxic fumes; contain fire runoff to prevent environmental contamination .