3-Isobutyl-1-methylxanthine / IBMX CAS 28822-58-4

1. 3-Isobutyl-1-methylxanthine (IBMX) is a potent, non-selective inhibitor of phosphodiesterases (PDEs), widely used in biomedical research to increase intracellular cAMP and cGMP levels by preventing their degradation .
2. IBMX CAS 28822-58-4 is a key component in adipocyte differentiation protocols, where it is used in combination with insulin and dexamethasone to induce the differentiation of preadipocytes (e.g., 3T3-L1 cells) into mature adipocytes .
3. 3-Isobutyl-1-methylxanthine is employed in studies of signal transduction, smooth muscle relaxation, and inflammation, and it acts as an antagonist at adenosine receptors (A1, A2A) .
4. Researchers utilize IBMX CAS 28822-58-4 as a research tool to investigate the roles of cyclic nucleotides in cellular processes, including cell proliferation, apoptosis, and gene expression .

1. 3-Isobutyl-1-methylxanthine must be stored in a tightly sealed container in a cool, dry, and well-ventilated environment; powder is stable for up to 3 years at -20°C or 2 years at 4°C .
2. For solution storage, prepare aliquots in tightly sealed vials and store at -20°C for up to 1 month, avoiding repeated freeze-thaw cycles to prevent degradation .
3. IBMX CAS 28822-58-4 is classified with GHS hazard statements H302 (Harmful if swallowed), H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation), with signal word "Warning" .
4. Personnel must wear chemical-resistant gloves, safety goggles with side-shields, and protective clothing when handling this substance, ensuring adequate ventilation in work areas .

1. Isolate the spill area, ensure adequate ventilation, and wear full personal protective equipment including chemical-resistant gloves, safety goggles, and suitable dust respirator when 3-Isobutyl-1-methylxanthine is released .
2. Collect IBMX CAS 28822-58-4 by sweeping or vacuuming without creating dust using non-sparking tools, and place into suitable closed containers for disposal .
3. Avoid creating dust clouds during cleanup to prevent respiratory exposure; prevent the product from entering drains or water courses .
4. Dispose of collected material according to local, state, and federal environmental regulations through licensed chemical destruction or controlled incineration .

1. 3-Isobutyl-1-methylxanthine is not classified as highly flammable but may decompose under fire conditions, emitting toxic fumes including carbon monoxide, carbon dioxide, and nitrogen oxides (NOx) .
2. Use water spray, dry chemical powder, carbon dioxide, or alcohol-resistant foam to extinguish fires involving the product; do NOT use a direct water jet as IBMX CAS 28822-58-4 may scatter burning material .
3. Firefighters should wear self-contained breathing apparatus in pressure-demand, MSHA/NIOSH approved, and full protective gear when handling burning 3-Isobutyl-1-methylxanthine due to hazardous decomposition gases generated by thermal decomposition .
4. During combustion, may emit irritant and toxic fumes; move containers from the fire area if safe to do so, and contain fire runoff to prevent environmental contamination .