6-O-Benzylguanine CAS 19916-73-5

1. 6-O-Benzylguanine is a guanine analogue with antineoplastic activity that functions as a potent, selective, and irreversible inhibitor of the DNA repair enzyme O⁶-alkylguanine-DNA alkyltransferase (AGT/MGMT) .
2. 6-O-Benzylguanine acts as a suicide substrate for AGT, transferring its benzyl group to the active-site cysteine residue of the enzyme, which permanently inactivates AGT and prevents 6-O-Benzylguanine CAS 19916-73-5 from repairing alkylation-induced DNA damage .
3. By depleting AGT activity, 6-O-Benzylguanine potentiates the cytotoxic effects of DNA-damaging alkylating agents such as temozolomide and BCNU, making 6-O-Benzylguanine CAS 19916-73-5 a valuable chemosensitizer in cancer research and clinical trials for gliomas and other malignancies .
4. In research applications, 6-O-Benzylguanine is widely used to study the role of AGT in chemotherapy resistance, to investigate DNA repair mechanisms, and to explore the efficacy of combination cancer therapies .

1. 6-O-Benzylguanine powder must be stored in a tightly sealed container at -20°C, protected from light and moisture, where it is stable for up to 3 years .
2. For solution storage, 6-O-Benzylguanine CAS 19916-73-5 can be dissolved in DMSO (up to 60 mg/mL) and stored at -20°C for up to 1 month or at -80°C for up to 6 months, avoiding repeated freeze-thaw cycles .
3. 6-O-Benzylguanine is classified with GHS hazard statements H315 (Causes skin irritation), H319 (Causes serious eye irritation), and H335 (May cause respiratory irritation), with signal word "Warning" .
4. Personnel must wear chemical-resistant gloves, safety goggles with side-shields, and protective clothing when handling 6-O-Benzylguanine, ensuring adequate ventilation in work areas .

1. Isolate the spill area, ensure adequate ventilation, and wear full personal protective equipment including chemical-resistant gloves, safety goggles, and a suitable dust respirator when 6-O-Benzylguanine is released .
2. Collect 6-O-Benzylguanine CAS 19916-73-5 by sweeping or vacuuming without creating dust using non-sparking tools, and place into suitable closed containers for disposal .
3. Avoid creating dust clouds during cleanup to prevent respiratory exposure; prevent 6-O-Benzylguanine from entering drains or water courses .
4. Dispose of collected material according to local, state, and federal environmental regulations through licensed chemical destruction or controlled incineration .

1. 6-O-Benzylguanine is not classified as highly flammable but may decompose under fire conditions, emitting toxic fumes including carbon monoxide, carbon dioxide, and nitrogen oxides (NOx) .
2. Use 6-O-Benzylguanine CAS 19916-73-5 water spray, dry chemical powder, carbon dioxide, or alcohol-resistant foam to extinguish fires involving 6-O-Benzylguanine .
3. Firefighters should wear self-contained breathing apparatus in pressure-demand, MSHA/NIOSH approved, and full protective gear when handling burning 6-O-Benzylguanine due to hazardous decomposition gases generated by thermal decomposition .
4. During combustion, 6-O-Benzylguanine may emit irritant and toxic fumes; contain fire runoff to prevent environmental contamination .